Recombinant Kinase Substrates
Field of Application
All kinase substrates can be used in a broad spectrum of biochemical kinase assay technologies including radiometric activity assays (e.g. Scintiplate®, filter binding assays), in ATP consumption or antibody-based assays. Substrates have been validated for the use in radiometric assays as well as with the ADP-Glo™ kinase assay technology from Promega Corp.
Our Products
| Product | Product Number | Data Sheet | Product Description | Used at PQ for FlashPlate Assays with | Used at PQ for Filter Binding Assays with | |
|---|---|---|---|---|---|---|
| ProQinase™ ATF2 | 0594-0000-2 |
|
human, internal fragment, GST-HIS fusion protein, expressed in E.coli |
JNK1, JNK2, JNK3, p38-alpha, p38-beta | p38-alpha, p38-beta, p38-delta, p38-gamma, JNK3 | |
| ProQinase™ CDCA8 | 1773-0000-1 |
|
human, full length, GST-HIS fusion protein, expressed in E.coli |
TTK | TTK | |
| ProQinase™ ELK1 | 1211-0000-1 |
|
human, full length, GST-HIS fusion protein, expressed in Sf9 cells |
ERK2 | ||
| ProQinase™ ERK2 K54R | 0416-0000-5 |
|
human, full length, mutationally inactivated (K54R), N-terminally HIS-tagged, expressed in E.coli |
MEK1 wt | MEK1 wt | |
| ProQinase™ JNK1 K55R/K56R | 0524-0000-1 |
|
human, full length, mutationally inactivated (K55R, K56R), HIS fusion protein, expressed in E.coli |
MKK7 | ||
| ProQinase™ JUN | 0718-0000-1 |
|
human, internal fragment, GST fusion protein, expressed in E.coli |
JNK1, JNK2 | ||
| ProQinase™ MEK1 K97M | 0785-0000-1 |
|
human, full length, mutationally inactivated (K97M), untagged, expressed in Sf9 cells |
B-RAF wt, B-RAF V600E, RAF1 Y340/Y341D | B-RAF wt, B-RAF V600E, RAF1 Y340/Y341D | |
| ProQinase™ p38alpha K53A | 0769-0000-1 |
|
human, full length, mutationally inactivated (K53A), N-terminally HIS-tagged, expressed in E.coli |
MKK6 S207D/T211D, SAK | MKK6 S207D/T211D | |
| ProQinase™ RB-CTF | 0040-0000-6 |
|
human, C-terminal fragment, GST fusion protein, expressed in E.coli |
MST1, MST2, MST4, NEK2 | CDK1/CycA, CDK1/CycB1, CDK1/CycE, CDK2/CycE, CDK3/CycE, CDK4/CycD1, CDK4/CycD3, CDK5/p25NCK, CDK5/p35NCK, CDK6/CycD1, CDK7/CycH/MAT1, CDK9/CycT, GSK3-beta, MST4, NEK6, NLK, PCTAIRE1, VRK1 | |
| ProQinase™ RB-S6P | 0339-0000-5 |
|
GST fusion protein, expressed in E.coli |
CDC42BPB, CLK3, MYLK2, ROCK2 | ||
| ProQinase™ RBER-CDC25tide | 0590-0000-1 |
|
GST fusion protein, expressed in E.coli |
PLK1, PLK3 | ||
| ProQinase™ RBER-CHKtide | 0581-0000-5 |
|
GST fusion protein, expressed in E.coli |
all CDKs, AMPK-alpha fl, ARK5, CHK1, COT, ERK1, ERK2, GSK3-beta, HIPK1, HIPK3, IKK-beta, MAPKAPK5, MARK1, MARK3, MELK, MINK1, NIK, p38-delta, p38-gamma, PCTAIRE1, PKC-mu, PLK1, RIPK2, TSK2, TTK, VRK1 | ERK1, HIPK3, MAPKAPK5, MELK, RIPK2, TTK | |
| ProQinase™ RBER-GSK3(14-27) | 0349-0000-5 |
|
GST fusion protein, expressed in E.coli |
ACV-R1, AKT1, AKT2, AKT3, DAPK1, IKK-epsilon, PIM1, PIM2, PRK1, S6K, SGK1, SGK3, SNK, TGFB-R1 | ||
| ProQinase™ RBER-IRStide | 0863-0000-1 |
|
GST fusion protein, expressed in E.coli |
CDK8/CycC, p38-gamma | CDK8/CycC | |
| ProQinase™ RBER-NTRK3tide | 1132-0000-1 |
|
GST fusion protein, expressed in E.coli |
FGF-R3, TRK-C |
Info in Brief
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Item Size
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100 μg, 1 mg, bulk
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Global Delivery
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Within 2 days after the receipt of a purchase order
US customers are served from our US site -
Suitability for ATP Consumption Assays
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Please refer to the certificates of analysis of the respective substrate for its suitability for use in ATP consumption based assays (like e.g. ADP-Glo™)
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Material Safety Data Sheet